In this episode of Listen In, we showcase cutting-edge mass spectrometric analysis of mucins, including the characterization of mucinases, enrichment techniques, and complete mucinomic mapping of translationally relevant mucin proteins.

Mucins are a diverse family of high molecular weight glycoproteins that play critical roles in protecting and lubricating epithelial surfaces, making them integral to numerous physiological processes and disease states.

As a promising class of biomarkers, mucins may offer unique insights into various pathologies, such as cancer and chronic inflammatory diseases. However, their complex structures and extensive glycosylation present significant challenges in their study and application.

Mucin-domain glycoproteins are densely O-glycosylated and play key roles in many biological functions.

However, their dense O-glycosylation remains enigmatic both in the glycoproteomic landscape and structural dynamics, primarily due to the challenges associated with studying mucin domains.

In this episode, you will get an introduction to the (glyco)proteomic techniques used in Mucinomics and learn how proper sample preparation of mucin-containing tissues for mass spectrometry is key to discovery.

MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.

Watch the full presentation here:

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode, learn about latest advancements in multiplex assay technology.

Hear from real user experience how the advanced xMAP INTELLIFLEX® DR-SE Instrument revolutionizes assay multiplexing, enabling simultaneous rapid data acquisition for multiple sample parameters to expand the depth and breadth of your research.

Watch the full presentation here: https://events.bitesizebio.com/user-experience-of-the-xmap

This compact, flow-based multiplex platform integrates xMAP® Technology with modern enhancements to streamline assay development, enhance user experience, and deliver fast, reliable results by acquiring data for two parameters per analyte simultaneously.

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Immunoassays are pivotal in scientific research, enabling the precise identification and measurement of specific analytes. They pave the way for investigations into disease mechanisms, discovering biomarkers, and evaluating the efficacy of potential therapeutic interventions.

The key reagents included in an immunoassay can significantly impact reliability and reproducibility, underscoring the importance of understanding critical components within immunoassay kits.

When selecting an immunoassay platform, it's essential to consider critical elements such as multiplexing capabilities and sensitivity needs as part of the experimental design.

Additionally, understanding the various assay formats and their data outputs can aid in the immunoassay selection process.

In this episode, immunoassay experts give practical guidance in these areas, empowering researchers to achieve more precise and meaningful results, thereby advancing knowledge and understanding in various scientific fields.

MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not For Use In Diagnostic Procedures.

Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-strategies-to

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Multicolor 3D fluorescent confocal microscopy presents significant challenges in image acquisition and data analysis. However, AI is making a massive impact on 3D spatial biology.

This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids.

See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals.

And get a showcase of how AIVIA supports Python-based image analysis for microscopists.

Watch the full presentation here: https://events.bitesizebio.com/ai-powered-3d-organoid-analysis

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings.

Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations.

Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method.

Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate.

Larger batches would require multiple analysts for a single-day turnaround time.

With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential.

Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements.

It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL.

Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

Learn more about the capabilities of MULTI-seq in single-cell sequencing in this episode of Listen In. Developed by the Gartner Lab at UCSF, this innovative multiplexing method increases sample and cell throughput while minimizing cost and improving your data quality.

From modest experiments to expansive 96-plex studies and beyond, MULTI-seq enables truly mechanistic studies. Hit play to explore the potential of this tool and how it could benefit your research.

Watch the full presentation here: https://events.bitesizebio.com/multiplexing-magic-how-multi-seq/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time.

Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy.

This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics.

In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity.

See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.

We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration.

You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems.

Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/

"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

In this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers.

See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia.

Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations.

You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context.

We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms.

Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/